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What is the second step in collecting a 24-hour urine specimen?

  1. Save all voiding for the next 24 hours

  2. Start with an empty bladder

  3. Discard the first voiding

  4. Label the specimen container

The correct answer is: Save all voiding for the next 24 hours

To collect a 24-hour urine specimen, the second step is to save all voiding for the next 24 hours. This means that all urine produced during this time period should be collected in a container for testing. Option B is incorrect because starting with an empty bladder would not provide an accurate sample for testing. Option C is incorrect because the first voiding should be included in the sample. Option D is incorrect because labeling the specimen container should be done at the end after all urine has been collected.